Candidatus liberibacter solanacearum libepsdocuments. The specificity and sensitivity of polymerase chain reaction pcr primers developed for candidatus liberibacter solanacearum and candidatus liberibacter psyllaurous were evaluated in. The complete genome sequence of candidatus liberibacter. Genetic diversity of cadidatus liberibacter solanacearum. Following the detection of tpp in western australia, there has been extensive testing of tpp and plant material for the candidatus liberibacter solanacearum bacteria. Irrigation australia latest news up to 375 gigalitres of southern murraydarling basin water could be unused, mick keelty says the murraydarling basin authoritys top cop believes there could be approximately 375 billion litres of unused water in the southern murraydarling basin. Pm100228 eppo a1 and a2 lists of pests recommended for regulation as quarantine pests 2019. There are multiple psyllid species present in the state of florida that look similar to the potato psyllid, thus identification of this vector can be difficult and should. General features of the candidatus liberibacter solanacearum genome and comparison to ca. A survey of candidatus liberibacter solanacearum in. Government news is australias independent online publication reporting on government in australia. The disease has been known in the indian subcontinent for over a century crawford, 1912, husain and nath, 1927.
Detection of candidatus liberibacter asiaticus causing citrus huanglongbing disease onuma ruangwong and angsana akarapisan department of plant pathology, faculty of agriculture, chiang mai university, chiang mai 50200, thailand ruangwong, o. Liberibacter solanacearum candidatus liberibacter solanacearum was discovered in 2008 by a nz team led by dr lia liefting maf. Members of the genus are plant pathogens mostly transmitted by psyllids. Candidatus liberibacter solanacearum is an economically important pathogen in.
An emerging pathogen infecting potato and tomato1 binoy babu, mathews l. Candidatusliberibactersolanacearum archives government. Transmission of candidatus liberibacter solanacearum in. Candidatus liberibacter solanacearum ponte project. The bacteria can be acquired by the insects in the nymphal stages and may be transmitted throughout the lifespan. Candidatus liberibacter solanacearum and its vector bactericera cockerelli. Candidatus liberibacter solanacearum the cause of zebra chip disease in potato. The accessions dated from the 1970s and the seed had been sourced worldwide.
The disease was first reported in the united states in 2004. An unknown liberibacter species was detected by pcr in an ornamental rutaceous tree, cape chestnut calodendrum capense, in south africa. Zebra chip disease of potato is caused by the bacterium candidatus liberibacter solanacearum clso and is a serious pest of potato in the americas and new zealand. Bactericera cockerelli is a vector of candidatus liberibacter solanacearum, the pathogen associated with zebra chip disease of potato solanales. Candidatus liberibacter solanacearum proteobacteria is an important pathogen of solanaceous crops solanales. However, they were imported through a supplier in italy. Candidatus liberibacter solanacearum and tpp agriculture. Candidatus liberibacter solanacearum calsol is a phloemlimited bacterium that is the causal agent of the potato disease zebra chip. Candidatus liberibacter solanacearum candidatus liberibacter solanacearum is a phloemlimited. Dp 21 diagnostic protocols for regulated pests dp 212 international plant protection convention 1. Haplotypes of candidatus liberibacter solanacearum. Pdf horizontal transmission of candidatus liberibacter.
Detection of the liberibacters is based on pcr amplification of their 16s rrna gene with specific primers. Candidatus liberibacter asiaticus and candidatus liberibacter americanus are transmitted by the adults of the citrus psyllid diaphorina citri kuwayana. Zebra chip candidatus liberibacter solanacearum clso candidatus liberibacter solanacearum clso is an exotic pest to australia. Rapid pest risk analysis for candidatus liberibacter. Because of the genomic and serological properties the bacterium was assigned as a subspecies of the african hlb species and given the name candidatus liberibacter africanus subsp. Rapid pest risk analysis for candidatus liberibacter solanacearum this document provides a rapid assessment of the risks posed by the pest to the uk in order to assist risk managers decide on a response to a new or revised pest threat. In the united states, hlb is now established in florida and has resulted in. Candidatus liberibacter solanacearum invasive species.
Documents about candidatus liberibacter solanacearum libeps number title download. Introduction the symptoms on peppers include stunted growth, and. Pm 7143 1 candidatus liberibacter solanacearum 2020 eppo. Invasive species list and scorecards for california. Candidiatus liberibacter solanacearum department of agriculture.
Candidatus liberibacter solanacearum pflanzengesundheit jki. Candidatus liberibacter solanacearum lso is bacterium transmitted by psyllids to solanaceae and apiaceae plants. Candidatus liberibacter solanacearum liefting et al. First report of candidatus liberibacter solanacearum in the united kingdom in the psyllid trioza anthrisci. Monitoring for a potential pathogen in florida potato and.
Solanaceae in north america and new zealand, and is the putative causal agent of. Anne nissinen luke, finland, in collaboration with colleagues from anses france, agricola villena spain, aro volcani israel, csicica spain, inra france and ivia spain. A photo gallery of symptoms associated with candidatus liberibacter solanacearum haplotypes d and e infections in different host plants, and their psyllid vectors, has been prepared by dr. Candidatus liberibacter solanacearum lso is a phloemlimited, gramnegative, unculturable bacterium that is primarily spread by psyllid insect vectors. Genomic sequence of candidatus liberibacter solanacearum ncbi. Liberibacter solanacearum in the canary islands and psyllid. Draft annex to ispm 27 candidatus liberibacter solanacearum page 2 of 42 international plant protection convention for the identification of candidatus liberibacter solanacearum diagnostic protocol as there is only one method and there are no decision points.
The fastidious nature of these pathogens has hindered the study. Zc, a newly emerging and economically important disease of potato worldwide. Candidatus liberibacter solanacearum clso was first described in connection with diseases of solanaceous crops, including potato, tomato. Haplotypes a and b are present in the americas andor new zealand, where they are vectored to solanaceous plants by the potato psyllid, bactericera cockerelli sulc hemiptera. Candidatus liberibacter solanacearum, associated with. We provide independent coverage of local, state and federal government and their administration of programs and initiatives. First report of candidatus liberibacter solanacearum in. From this, only an alignment to a bp segment of the hyv i gene was indicated. The first two are associated with zebra chippsyllid yellows of potatoes and other solanaceous plants, vectored by the tomatopotato psyllid bactericera cockerelli in north and central america and new zealand. Specific scope this standard describes a diagnostic protocol for candidatus liberibacter solanacearum, and for its detection in the psyllid. This document is pp320, one of a series of the plant pathology department, ufifas extension. Candidatus liberibacter is a genus of gramnegative bacteria in the rhizobiaceae family.
Pra record for candidatus liberibacter solanacearum in solanaceae. Pcrbased diagnostic methods for candidatus liberibacter. Candidatus liberibacter solanacearum haplotypes d and e. Candidatus liberibacter solanacearum infects pepper plants. The occurrence of candidatus liberibacter solanacearum lso was determined in commercial and wild species of the family apiaceae, obtained from three seed collections held in the united kingdom. Calsol, jumping plant lice, lso, psylloidea, vector created date.
Candidatus liberibacter solanacearum calsol associated. Detection, distribution, and genetic variability of. A diagnostic protocol for liberibacter africanus, liberibacter americanus and liberibacter asiaticus and for their detection in their psyllid vectors diaphorina citri and trioza erytreae has been published by eppo 2014. Pest information candidatus liberibacter solanacearum is a phloemlimited, gramnegative, unculturable bacterium that is associated with several emerging diseases. Tomato potato psyllid tpp can carry a bacterium called candidatus liberibacter solanacearum sometimes known as clso or calsol which is associated with the zebra chip disease in potatoes. Although only small quantities of each seed accession was tested, a high proportion of commercial. Candidatus liberibacter solanacearum, associated with plants in the family solanaceae lia w. The disease was first seen in potato in mexico and parts of central america in the 1990s and then was spread to southern states of the us by its vector, the tomatopotato psyllid bactericera cockerelli and also into new zealand possibly with. The term candidatus indicates that it has not proved possible to maintain this bacterium in culture. First report of candidatus liberibacter solanacearum in carrot in france. Candidatus liberibacter solanacearum associated with the.
Exclusion of infected seeds is mandatory to prevent the introduction and the establishment of calsol in new areas, such as italy. Candidatus liberibacter solanacearum libepsreporting. Candidatus liberibacter solanacearum lso has emerged as a serious threat worldwide. It is considered very invasive due to its ability to be transported primarily in infective psyllids munyaneza et al.
Bactericera cockerelli and candidatus liberibacter solanacearum. Candidatus liberibacter solanacearum calsol associated with apiaceous crops current situation a bacterial plant pathogen, candidatus liberibacter solanacearum calsol, has been detected in the italian giant variety of imported parsley seed. Candidatus liberibacter solanacearum is found in all states west of the rocky mountains, kansas and in texas. Transmission of candidatus liberibacter solanacearum in carrot seeds article pdf available in plant pathology 642 may 2014 with 1,921 reads how we measure reads.
This study assessed the potential economic impacts of the invasion of the plant pathogenic bacterium candidatus liberibacter solanacearum. Candidatus liberibacter solanacearum detected on potatoes in idaho, oregon and. Clpsy was not described and validly published, and no reference material is available, while clso was validly published and reference material is available 21,32,44,55. Risk assessment of candidatus liberibacter solanacearum. Candidatus liberibacter solanacearumpositive psyllidspecimenstotal of analyzed specimens total captured b the three positive specimens belong to bactericera sp. A rapid field detection system for citrus huanglongbing. The protocol involves detection based on the disease symptoms and molecular tests pcr, and reporting and documentation. Candidatus liberibacter solanacearum calsol is an emerging bacterium, which is seedborne in carrot bertolini et al. Three haplotypes of the recently discovered bacterium species candidatus liberibacter solanacearum are described and related to geographic ranges. A new liberibacter species, candidatus liberibacter solanacearum lso recently associated with the potatotomato psyllidtransmitted diseases in tomato and capsicum in new zealand liefting et al. It was subsequently found to be the cause of the debilitating potato disease zebra chip that has affected the usa industry since 2000. Detection of candidatus liberibacter asiaticus causing. Liberibacter solanacearum 21 be the preferred species name.